PS / KCl INDUCED BLADDER INFLAMMATION

A MODEL FOR PAINFUL BLADDER SYNDROME (INTERTISTIAL CYSTITIS)

Cystometry in anesthetized animals after PS / KCl exposition.

Intravesical infusion of protamine sulfate and KCl in rodents produces bladder inflammation and bladder hyperreflexia mimicking some pathological features of human Painful Bladder Syndrome (PBS) also known as interstitial cystitis.

Rat

• This model is suitable for testing compounds for effects on the increased frequency and decreased bladder capacity associated with PBS.
• This model is often used as secondary model to the isovolumetric model.
• Compounds that show a positive response in this model include NK1 receptor antagonists and inhibitor of TNF-α interferon γ and interleukine production.

• Cystometry was performed in anesthetized female rats.
• Intravesical infusion of Nacl 0.9% was performed followed by intravesical infusion of PS and KCl.
• Test compounds can be administered via various routes (i.v., i.p., p.o., s.c. or by osmotic pumps) and cystometric parameters evaluated for up to two hours post-administration.

• Bladder capacity
• Intercontraction intervals during continuous cystometry
• Micturition pressure
• Micturition volume
• Basal intravesical pressure
• Threshold pressure for micturition

• Chuang YC et al, Urology 61( 3): 664-70, 2003
• Tyagi P et al, BJU Int 104 (11):1689-92, 2009