ACUTE CYCLOPHOSPHAMIDE INDUCED URINARY BLADDER INFLAMMATION

A MODEL FOR INTERSTITIAL CYSTITIS/PAINTFUL BLADDER SYNDROME

MODEL

Evaluation of inflammatory mediators in an acute cystitis model.

Cyclophosphamide-induced cystitis is a well-characterized model of subacute, inflammatory  visceral pain in rodents. This preclinical model is usually associated with urinary   bladder inflammation, bladder overactivity and referred mechanical pain.

SPECIES

 Rat.

INTEREST

This is a model of acute urinary bladder inflammation, characterized by high levels of pro-inflammatory cytokines, chemokine and adhesion molecules in the urinary bladder, and high levels of lipid derived mediators in urines.

Compounds that produce positive effects in this model   include: non-steroidal anti-inflammatory drugs (aspirin and ibuprofen).

MODEL DESCRIPTION

Urinary bladder inflammation is assessed at different time points after intraperitoneal injection of cyclophosphamide.

Inflammatory mediators are quantified in urinary bladders and urines using Multiplex technology, EIA or ELISA assays.

Compounds can be administered via various routes (i.v., i.p., s.c., p.o.).  

PARAMETERS EVALUATED

Inflammatory parameters: tissue wet weight, bladder wall thickness, macroscopic damage (edema and hemorrhage), microscopic analysis.

Inflammatory mediators: arachidonic acid metabolites (PGE2, lipoxin A4, 15-epi lipoxin), pro-inflammatory cytokines (IL-1b, IL-6), chemokines, adhesion molecules (in collaboration with Ambiotis and UMR1043 Inserm).

UROsphere can also evaluate the feasibility of additional biomarkers to meet clients’ needs.

 SCIENTIFIC PUBLICATIONS

Ahluwalia A et al, Br J Pharmacol 111: 1017-1022, 1994

Boucher M et al, J Urol 164: 203-208, 2000

Gray KJ et al, J Urol 136: 497-500, 1986