CYCLOPHOSPHAMIDE-INDUCED HYPERACTIVE BLADDER MODEL

A MODEL OF PAINFUL BLADDER SYNDROME (INTERSTITIAL CYSTITIS)

Cystometry in conscious animals after cyclophosphamide treatment.

Cyclophosphamide treatment in rodents produces bladder inflammation and disruption of the urothelium resulting in abdominal pain and bladder overactivity.

Rat, mouse, guinea-pig

• This model is suitable for testing compounds for effects on the increased frequency and decreased bladder capacity associated with painful bladder syndrome/interstitial cystitis.
• Compounds that show a positive response in this model include aspirin, COX inhibitors and bradykinin B2 receptor antagonists.

• Cystometry in restraining or metabolic cages is performed in conscious animals 24 hours after intraperitoneal injection of cyclophosphamide.
• Test compounds can be administered via various routes (i.v., i.p., p.o., s.c., i.g. or by osmotic pumps) and cystometric parameters evaluated for up to two hours post-administration.

• Bladder capacity
• Intercontraction intervals during cystometry
• Micturition pressure
• Micturition volume
• Basal intravesical pressure
• Threshold pressure for micturition

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