PS / LPS INDUCED BLADDER INFLAMMATION

A MODEL FOR PAINFUL BLADDER SYNDROME (INTERSTITIAL CYSTITIS)

Cystometry in anesthetized animals after PS / LPS treatment.

Intravesical instillation of protamine sulfate and lipolysaccharide in rodents produces bladder inflammation and bladder hyperreflexia mimicking some of the pathological features of human Painful Bladder Syndrome (PBS), also known as interstitial cystitis.

Rat, mouse, guinea-pig

• This model is suitable for testing compounds for effects on increased frequency and decreased bladder capacity associated with PBS.
• Compounds that show a positive response in this model include NK₁ receptor antagonists and inhibitors of TNF-α, interferon γ and interleukine production.
   

• Intravesical instillation of a protamine sulfate (PS) solution followed by a lipopolysaccharide (LPS) solution.
• Cystometry is performed in anesthetized animals following PS/LPS treatment.
• Test compounds can be administered via various routes (i.v., i.p., p.o., i.g. or s.c.) and cystometric parameters evaluated up to two hours post-administration.  
   

• Bladder capacity
• Intercontraction intervals during cystometry
• Micturition pressure
• Micturition volume
• Basal intravesical pressure
• Threshold pressure for micturition

• Saban MR et al, Am J Physiol Renal Physiol 282 : F202-F202-210, 2002
• Lecci A et al, J Urol 160: 206-209, 1998
• Gonzalez RR et al, J Urol 173: 630-34, 2005